Optimization and Validation of a Diagnostic Real-Time PCR for Bovine Brucellosis
نویسندگان
چکیده
منابع مشابه
Checklist for optimization and validation of real-time PCR assays.
Real-time polymerase chain reaction (PCR) is a frequently used technique in molecular diagnostics. To date, practical guidelines for the complete process of optimization and validation of commercial and in-house developed molecular diagnostic methods are scare. Therefore, we propose a practical guiding principle for the optimization and validation of real-time PCR assays. Based on literature, e...
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Real time RT-PCR is the most sensitive method for quantitation of gene expression levels. The accuracy can be dependent on the mathematical model on which the quantitative methods are based. The generally accepted mathematical model assumes that amplification efficiencies are equal at the exponential phase of the reactions for the same amplicon. However, no methods are available to test the ass...
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ژورنال
عنوان ژورنال: Advances in Animal and Veterinary Sciences
سال: 2015
ISSN: 2307-8316
DOI: 10.14737/journal.aavs/2015/3.11.577.587